CD36, Human, mAb FA6-152 Antibody

SKU: orb1108941

Description

Mouse monoclonal antibody to CD36

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, Functional Studies, IA, IF, IHC-Fr, IP
• Dilution Range: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Human
• Application Notes:
  F: Tissue embedded in tissue-tek (for instance aortic tissue) followed by freezing in liquid nitrogen; 7-8 um sections; air-dried; aceton-fixed; 10 % NGS as block (Ref 4). FC: Antibody FA6-152 stains the extracellular domain of CD36. Unfixed cells;2 ug per 100.000 cells. Positive on granulocytes (Ref 1). FS: Platelet aggregation and secretion was induced by; 1µg/ml antibody (Ref 2). IA: 10 µg/ml antibody as coat diluted in Tris-buffered saline ; 100 um;l/well; o/n at RT (Ref 3). IF: unfixed cells were incubated for 30 minutes at 4 °C followed by a secondary FITC polyclonal antibody; one-minute methanol fixation before analysis (Ref 1). IP: 88 kDa sialoglycoprotein in platelets; 85 and 88 kDa in HEL cells. 10 ug antibody/200 ug protein

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: 20-Weeks-old fetal erythrocytes
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Buffer/Preservatives: Quantities >500 µg will be sent without preservatives and are carrier-free.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPIV, Fatty acid translocase (FAT), platelet glycoprotein 4, PAS IV, Platelet collagen receptor, thrombospondin receptor Gene name: CD36