CD32 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499307

Description

CD32 Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Markers, Immunology, Stem Cells

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:200-400, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R8F6
• Immunogen: KLH conjugated synthetic peptide derived from human CD32
• Target: FCGR2B
• Molecular Weight: 31 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD32 antigen; CDw32; CD32B; Fc fragment of IgG, low affinity II, receptor for(CD32); Fc fragment of IgG, low affinity IIb, receptor; Fc fragment of IgG, low affinity IIb, receptor(CD32); Fc fragment of IgG, low affinity IIb, receptor for(CD32); Fc fragment of IgG, low affinity IIb, receptor for; Fc gamma receptor IIB; Fc gamma RII; Fc gamma RIIB; Fc-gamma RII; Fc-gamma RII-b; Fc-gamma-RIIb; FCG2; FCG2B_HUMAN; FCGR2B; FCRII; FcRII-b; IgG Fc receptor II beta; IgG Fc receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lymphocyte antigen 17.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Human placenta, 2: Human liver, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 34 kDa, Observed MW: 34 kDa.

Tissue: Human liver, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499307.

Tissue: Human placenta, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for.

Quick Database Links

Gene Symbol

FCGR2B

UniProt

P31994