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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | E.coli |
|---|---|
| Tag | His-tag |
| Expression Region | pet-22b(+) |
| Purification | Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). |
| MW | ~89kDa |
| Protein Sequence | AGCAGCCTGGGCGGTACCGATAAAGAACTGCGTCTGGTGGATGGTGAAAATAAATGCAGTGGTCGTGTGGAAGTGAAAGTTCAGGAAGAATGGGGCACCGTGTGTAATAATGGTTGGAGCATGGAAGCCGTTAGCGTGATCTGTAATCAGCTGGGCTGCCCGACCGCAATTAAAGCCCCGGGCTGGGCCAATAGTAGTGCAGGCAGTGGCCGTATCTGGATGGATCATGTTAGCTGCCGTGGCAATGAAAGCGCCCTGTGGGATTGTAAACATGATGGTTGGGGCAAACATAGTAATTGTACCCATCAGCAGGATGCCGGTGTTACCTGTAGTGATGGCAGTAATCTGGAAATGCGTCTGACCCGCGGTGGCAATATGTGCAGTGGCCGTATTGAAATTAAATTCCAGGGCCGCTGGGGCACCGTGTGTGATGATAACTTCAATATTGATCATGCAAGTGTTATCTGTCGTCAGCTGGAATGTGGTAGCGCCGTTAGCTTCAGTGGCAGTAGTAACTTCGGTGAAGGCAGTGGTCCGATCTGGTTCGATGATCTGATCTGTAATGGTAATGAAAGTGCCCTGTGGAATTGTAAACATCAGGGTTGGGGTAAACATAATTGTGATCATGCCGAAGATGCCGGTGTGATCTGTAGTAAAGGCGCAGATCTGAGTCTGCGTCTGGTTGATGGTGTGACCGAATGCAGTGGTCGCCTGGAAGTTCGCTTCCAGGGTGAATGGGGTACCATCTGTGATGATGGTTGGGATAGCTATGATGCAGCAGTTGCCTGTAAACAGCTGGGCTGTCCGACCGCAGTGACCGCCATTGGTCGCGTGAATGCAAGTAAAGGCTTCGGTCATATCTGGCTGGATAGCGTTAGCTGCCAGGGCCATGAACCGGCAATCTGGCAGTGCAAACATCATGAATGGGGCAAACACTATTGTAATCATAATGAAGATGCCGGCGTGACCTGTAGTGACGGTAGTGATCTGGAACTGCGTCTTCGCGGTGGTGGCAGTCGTTGTGCAGGTACCGTTGAAGTTGAAATTCAGCGTCTGCTGGGTAAAGTGTGTGATCGCGGCTGGGGTCTGAAAGAAGCAGATGTTGTGTGCCGCCAGCTGGGTTGTGGCAGCGCCCTGAAAACCAGTTATCAGGTGTATAGTAAAATCCAGGCAACCAATACCTGGCTGTTCCTGAGCAGTTGTAATGGTAACGAAACCAGTCTGTGGGATTGCAAAAATTGGCAGTGGGGTGGCCTGACCTGTGATCATTATGAAGAAGCCAAAATTACCTGTAGCGCACATCGCGAACCGCGCCTGGTTGGCGGCGATATTCCGTGCAGTGGCCGCGTTGAAGTTAAACATGGTGATACCTGGGGCAGTATCTGTGATAGCGACTTCAGTCTGGAAGCAGCCAGCGTTCTGTGTCGCGAACTGCAGTGTGGCACCGTTGTTAGTATTCTGGGCGGCGCACACTTCGGCGAAGGCAATGGTCAGATCTGGGCAGAAGAATTCCAGTGTGAAGGTCATGAAAGCCATCTGAGTCTGTGTCCGGTTGCACCGCGTCCGGAAGGCACCTGCAGCCATAGTCGCGATGTTGGTGTTGTGTGTAGCCGCTATACCGAAATTCGTCTGGTTAATGGCAAAACACCTTGCGAAGGTCGTGTTGAACTGAAAACCTTAGGTGCCTGGGGTAGCCTGTGCAATAGTCATTGGGATATTGAAGATGCCCATGTTCTGTGCCAGCAGCTGAAATGCGGTGTTGCACTGAGTACCCCGGGCGGCGCACGCTTCGGTAAAGGTAATGGCCAGATCTGGCGTCACATGTTCCATTGCACCGGCACCGAACAGCACATGGGCGATTGTCCGGTTACCGCCCTGGGTGCCAGCCTGTGTCCGAGCGAACAGGTGGCAAGCGTGATCTGTAGCGGCAATCAGAGTCAGACCCTGAGCAGCTGCAATAGTAGCAGCCTGGGTCCGACCCGTCCGACCATTCCGGAAGAAAGCGCCGTGGCCTGCATTGAAAGCGGTCAGCTGCGTCTGGTCAATGGTGGCGGTCGTTGTGCCGGTCGTGTGGAGATCTATCATGAAGGCAGTTGGGGTACCATCTGTGACGATAGTTGGGATCTGAGCGATGCCCATGTGGTGTGTCGTCAGTTAGGTTGCGGCGAAGCCATTAATGCAACCGGTAGCGCACACTTCGGTGAAGGTACCGGTCCGATCTGGCTGGACGAAATGAAATGCAATGGTAAAGAAAGCCGTATCTGGCAGTGTCATAGCCATGGTTGGGGCCAGCAGAATTGCCGCCATAAAGAAGATGCAGGTGTTATCTGTAGCGAATTCATGAGCCTGCGCCTGACCAGTGAAGCCAGTCGCGAAGCATGCGCCGGCCGTCTGGAAGTGTTCTATAATGGTGCCTGGGGCACCGTT |
Storage & Handling
−| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 4M Urea, pH 7.4 |
| Concentration | > 0.5mg/ml |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protein Handling and Storage Guide
Protein Handling Guide
CD163 protein (orb763326)
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