CD151 Rabbit Polyclonal Antibody

SKU: orb585670

Description

Rabbit polyclonal antibody to CD151

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Target: CD151
• Molecular Weight: 28kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24

Application: IHC, Species+tissue/cell type:Human nasal epithelial cells, Primary antibody dilution: 1:1000, Secondary Antibody: Goat anti-rabbit Alexa Fluor 546, Secondary antibody dilution: 1:1000.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-CD151 Antibody, Titration: 1.0 ug/ml, Positive Control: A549 Whole Cell. CD151 is supported by BioGPS gene expression data to be expressed in A549.

Quick Database Links

Gene Symbol

CD151

UniProt

P48509

RefSeq (Protein):NP_620599