CBFA2T2 Antibody (N-term)

SKU: orb1935033

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:2000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB35359
• Target: This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2.
• Molecular Weight: 67133 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85, CBFA2T2, EHT, MTGR1

All lanes: Anti-CBFA2T2 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). CBFA2T2 immunoreactivity is localized to Nucleus significantly.

Quick Database Links

Gene Symbol

This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2.

UniProt

O43439

RefSeq (Protein):NP_005084.1, NP_001028171.1, NP_001034798.1