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Catalog Number | orb1539145 |
---|---|
Category | Antibodies |
Description | CAV3/Caveolin 3 Antibody |
Target | CAV3 / Caveolin 3 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Bovine, Canine, Equine, Human, Monkey |
Concentration | 0.5 mg/ml |
Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Immunogen | Peptide with sequence EHTDLEAQIVKDIH-C, from the N-Terminus of protein sequence according to NP_203123.1. |
Tested applications | ELISA, IHC, IHC-P, WB |
Dilution range | IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:4000), WB (0.1 - 0.3 µg/ml) |
Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx. 23kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CAV3, Caveolin-3, Caveolin3, Caveolin 3, LGMD1C, V Read more... |
Note | For research use only |
Goat Anti-CAV3 Antibody (0.3 µg/ml) staining of Mouse (A) and Rat (B) Heart lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
ELISA, IHC, WB | |
Bovine, Canine | |
Human, Mouse, Porcine, Rat | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |