Cav3.2 Antibody

SKU: orb67393

Description

Mouse Anti-Human Cav3.2 Monoclonal IgG1

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-303 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N55/10 (Formerly sold as S55-10)
• Immunogen: Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2
• Target: Cav3.2
• Molecular Weight: 260kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2, CACNA1H, KIAA1120, Cav3.2, CACNA1HB, calcium channel, voltage-dependent, T type, alpha 1H subunit, alpha 1Hb subunit, ECA6, EIG6, FLJ90484, Low-voltage-activated calcium channel alpha1 3.2 subunit, low-voltage-activated calcium channel alpha13.2 subunit, voltage dependent t-type calcium channel alpha-1H subunit, voltage-dependent T-type calcium channel subunit alpha-1H, voltage-gated calcium channel alpha subunit Cav3.2, voltage-gated calcium channel alpha subunit CavT.2

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone N55/10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone N55/10. Tissue: frozen brain section. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10. Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

Quick Database Links

Gene Symbol

Cav3.2

UniProt

O95180

NCBI Gene ID

8912

RefSeq (Protein):NP_001005407.1