Cat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb346851
• Category: Antibodies
• Description: Cat IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: AP
• Reactivity: Feline
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Cat IgG, and Cat Serum.
• Immunogen: Cat IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:2,000 - 1:6,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Anti-Cat IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Goat Anti-Cat IgG Antibody Alkaline Phosphatase Co
• Note: For research use only

ELISA of Purified Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Cat IgG. Each well was coated in duplicate with 10 µg of Cat IgG (p/n orb2652776). The working dilution of Cat IgG Alk Phos is 1:2000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and NPP Substrate.