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Cat IgG F(ab')2 Antibody Fluorescein Conjugated
Description
Images & Validation
−
| Tested Applications | FC, FLISA, IF |
|---|---|
| Dilution Range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Feline |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Cat IgG F(ab')2 fragment |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Cat IgG, Cat IgG F(ab’)2 and Cat Serum. No reaction was observed against Cat IgG F(c). |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 10.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Cat IgG F(ab')2 Fluorescein Antibody [orb346029]
FITC
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin.
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Protocol Information
Cat IgG F(ab')2 Antibody Fluorescein Conjugated (orb346570)
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