PRC1 Antibody

SKU: orb344590

Description

Carboxypeptidase Y antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 10,000 - 1:42,000, WB: 1:5,000
• Reactivity: Yeast
• Application Notes:
  Carboxypeptidase Y Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Carboxypeptidase Y [Baker’s Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:42,000 is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carboxypeptidase Y [Baker’s Yeast]
• Target: PRC1
• Purity: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Western Blot of Rabbit anti-Carboxypeptidase Y (Baker's Yeast) antibody. Lane 1: cellular extracts. Lane 2: none. Load: 10 µg per lane. Primary antibody: Carboxypeptidase Y antibody at 1:5000 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG [H&L] secondary antibody at 1:4000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61 kDa, 61 kDa for Carboxypeptidase Y. Other band(s): none.

Western Blot of Rabbit anti-Carboxypeptidase Y Antibody. Lane 1: Carboxypeptidase Y. Load: 50 ng per lane. Primary antibody: Carboxypeptidase Y primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 53 kDa, 57 kDa for Carboxypeptidase Y. Other band(s): None.

Quick Database Links

Gene Symbol

PRC1

UniProt

P00729

RefSeq (Protein):NP_014026.1