PRC1 Antibody

SKU: orb344589

Description

Carboxypeptidase Y antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 10,000 - 1:42,000, WB: 1:5,000
• Reactivity: Yeast
• Application Notes:
  Carboxypeptidase Y Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Carboxypeptidase Y [Baker’s Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:42,000 is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carboxypeptidase Y [Baker’s Yeast]
• Target: PRC1
• Purity: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Western Blot of Rabbit anti-Carboxypeptidase Y (Baker's Yeast) antibody. Lane 1: cellular extracts. Lane 2: none. Load: 10 µg per lane. Primary antibody: Carboxypeptidase Y antibody at 1:5000 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG [H&L] secondary antibody at 1:4000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61 kDa, 61 kDa for Carboxypeptidase Y. Other band(s): none.

Western Blot of Rabbit anti-Carboxypeptidase Y Antibody. Lane 1: Carboxypeptidase Y. Load: 50 ng per lane. Primary antibody: Carboxypeptidase Y primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 53 kDa, 57 kDa for Carboxypeptidase Y. Other band(s): None.

Quick Database Links

Gene Symbol

PRC1

UniProt

P00729

RefSeq (Protein):NP_014026.1