PRC1 Antibody (Biotin)

SKU: orb344757

Description

Carboxypeptidase Y antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Yeast
• Application Notes:
  Anti-Carboxypeptidase Y Biotin Antibody has been tested by ELISA and Western Blot and is assayed against 1.0 ug of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carboxypeptidase Y [Baker’s Yeast]
• Target: PRC1
• Purity: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Carboxypeptidase Y antibody biotin conjugation, biotin conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Western Blot of Rabbit Anti-Carboxypeptidase Y Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Carboxypeptidase Y. Primary Antibody: Anti-Carboxypeptidase Y Antibody Biotin Conjugated at 1:1000 overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~60kDa.

Quick Database Links

Gene Symbol

PRC1

UniProt

P00729

RefSeq (Protein):NP_014026.1