CAPG Antibody

SKU: orb1248221

Description

CAPG Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.1-0.3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KAQVEIVTDGEEP
• Target: CAPG
• Molecular Weight: Approx 38kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAPG, capping protein (actin filament), gelsolin-like, AFCP, MCP, actin regulatory protein CAP-G, actin-regulatory protein CAP-G, gelsolin-like capping protein, macrophage capping protein, macrophage-capping protein

orb1248221 (0.3 ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CAPG (0.3 ug/ml) staining of Periheral Blood Lymphocytes (orb1248220 lane A), orb1248221 lane B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248221 (0.1 ug/ml) staining of Mouse (A) and Rat Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248221 (0.3 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CAPG

UniProt

P40121

RefSeq (Protein):NP_001738.2