Cadherin 17 / CDH17 antibody

SKU: orb2276346

Description

Cadherin 17 / CDH17 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A strong CDH17 staining should be seen in all epithelial cells. Negative Control: Colon: Non-epithelial cells should not show any CDH17 immunostaining. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b kappa
• Clone No.: Biorbyt-517M
• Immunogen: Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

BILL-cadherin; Cadherin-17; CDH17; HPT-1 cadherin; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1 (HPT-1); Intestinal peptide-associated transporter HPT-1; LI-cadherin (liver-intestine); Liver Cadherin; Liver-intestine cadherin

Colon Colorectal adenocarcinoma with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.

Ileum Neuroendocrine tumor with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.

Rectum mucosa A strong membranous CDH17 staining occurs in all epithelial cells of the rectum mucosa CDH17 immunohistochemistry.

Quick Database Links

UniProt

Q12864