Cadherin 17 / CDH17 antibody (MSVA-517M)

• Catalog Number: orb2276345
• Category: Antibodies
• Description: Cadherin 17 / CDH17 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-517M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2b kappa
• Immunogen: Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q12864
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BILL-cadherin; Cadherin-17; CDH17; HPT-1 cadherin;
• Note: For research use only
• Application notes: Positive Control: Colon: A strong CDH17 staining should be seen in all epithelial cells. Negative Control: Colon: Non-epithelial cells should not show any CDH17 immunostaining. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colon Colorectal adenocarcinoma with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.

Ileum Neuroendocrine tumor with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.

Rectum mucosa A strong membranous CDH17 staining occurs in all epithelial cells of the rectum mucosa CDH17 immunohistochemistry.