CAD Antibody (Center)

• Catalog Number: orb1938442
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P27708
• MW: 242984 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:25, FC: 1:25
• Clone Number: RB18384
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CAD protein, Glutamine-dependent carbamoyl-phospha
• Note: For research use only
• NCBI: NP_004332.2

All lanes: Anti-CAD Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: SW620 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 243 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CAD Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 243 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CAD Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 243 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the CAD antibody detected the CAD protein (arrow).

Staining CAD in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.