CA2 Antibody

• Catalog Number: orb1263325
• Category: Antibodies
• Description: CA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: CA2
• UniProt ID: P00918
• NCBI: P00918
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Carbonic anhydrase 2, Carbonate dehydratase II, Ca
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody at 1:1000 at each lane.

Western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35 ug/lane).

Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.