C5a/C5a des-Arg, Human, mAb 2952 Antibody

SKU: orb1108983

Description

Mouse monoclonal antibody to C5a/C5a des Arg

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: Functional Studies, IA, IHC-Fr, WB
• Dilution Range: For immunohistology, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. IHC-P: No pretreatment was used on human tissue sections. Antibody was used at a concentration of 1 μg/ml. As positive control human granulocytes were used. IHC-F: Tissue sections were fixed in Histochoice MB (Amresco) and blocked with DakoCytomation Protein Block. Pretreatment with levamisole solution was performed to quench endogenous phosphatases. As positive control drusen eye sections was used and as negative control sections lacking the first antibody (Ref.4). FS: Antibody 2952 functions as a neutralizing antibody blocking the interaction between C5a and its receptor. The antibody was functionally tested by competitive binding studies using [125I]C5a and Bt2-cAMP differentiated U937 cells (Ref.2).The biological activity can be defined as the ability of antibody to displace C5a from U937 cell surface. Positive control Human Granulocytes. General comment: Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products (ref.5).
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only