SCFR Antibody / c-Kit / CD117

SKU: orb1716223

Description

c-Kit, also called CD117, SCFR and Stem Cell Factor Receptor is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-SCFR has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-SCFR is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: 2-4ug/ml (order Ab without BSA for coating),Immunohistochemistry (FFPE): 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the SCFR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: C117/7119
• Immunogen: A portion of amino acids 50-250 from the human protein was used as the immunogen for the SCFR antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human gastrointestinal stromal tumor (GIST) with SCFR antibody (clone C117/7119) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P10721