c-Kit Antibody / CD117

• Catalog Number: orb639532
• Category: Antibodies
• Description: This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2c, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A full length recombinant human protein was used as the immunogen for the CD117 antibody.
• UniProt ID: P10721
• Tested applications: ELISA
• Dilution range: ELISA (order BSA/sodium azide-free format for coating)
• Application notes: Optimal dilution of the CD117 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: KIT/2674
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Epigenetics
• Note: For research use only

IHC staining of FFPE human GIST (gastrointestinal stromal tumor) with CD117 antibody (clone KIT/2674). HIER: boil tissue sections in pH9.5 1mM EDTA buffer for 10-20 min followed by cooling at RT for 20 min.