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Item 1 of 3
BTG1 Antibody (Center)
Catalog Number: orb1930662
| Catalog Number | orb1930662 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB26174 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 19209 Da |
| Target | This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1. |
| UniProt ID | P62324 |
| NCBI | NP_001722.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Protein BTG1, B-cell translocation gene 1 protein, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
BTG1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTG1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
(LEFT) Western blot analysis of BTG1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane).BTG1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of BTG1 Antibody (Center) in mouse NIH-3T3 tissue lysates (35 ug/lane).BTG1 (arrow) was detected using the purified Pab.
All lanes: Anti-BTG1 Antibody (Center) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
