BTG1 Antibody (Center)

SKU: orb1930662

Description

BTG1 Antibody (Center)

Research Area

Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 19209 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

BTG1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTG1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

(LEFT) Western blot analysis of BTG1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane).BTG1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of BTG1 Antibody (Center) in mouse NIH-3T3 tissue lysates (35 ug/lane).BTG1 (arrow) was detected using the purified Pab.

All lanes: Anti-BTG1 Antibody (Center) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P62324