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Catalog Number | orb749405 |
---|---|
Category | Antibodies |
Description | It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | BRD469 |
Tested applications | IHC-P |
Reactivity | All |
Isotype | Mouse IgG1, kappa |
Immunogen | Bromodeoxyuridine conjugated to KLH was used as the immunogen. |
Dilution range | Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This BrdU antibody is available for research use only. |
Storage | Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC. |
Expiration Date | 12 months from date of receipt. |
IHC-P | |
All | |
Mouse | |
Monoclonal | |
Unconjugated |
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