BRD4 Antibody

SKU: orb1258209

Description

BRD4 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein of human BRD4
• Target: BRD4
• Molecular Weight: Observed: 200kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRD4, CAP, HUNKI, MCAP, HUNK1

Western blot analysis of extracts of various cell lines, using BRD4 antibody (orb1258209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of U-2 OS cells using BRD4 Polyclonal Antibody (orb1258209) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

BRD4

UniProt

O60885