Bradykinin (acetate)

SKU: orb1306277

Description

Bradykinin (acetate) is a bioactive peptide generated by the kallikrein-kinin system, functioning as a key inflammatory and neuromodulatory mediator. It is a potent vasodilator involved in vascular and renal regulation, widely used in cardiovascular, inflammation, and pain research in both in vitro and in vivo experimental models.

Research Area

Metabolism Research, Pharmacology & Drug Discovery, Protein Biochemistry

Key Properties

• CAS Number: 6846-03-3
• MW: 1120.26
• Purity: >99.99% (May vary between batches)
• Formula: C₅₂H₇₇N₁₅O₁₃
• SMILES: CC(O)=O.N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O
• Target: Endogenous Metabolite,Bradykinin Receptor,Serine Protease
• Solubility: DMSO:10 mM

Bioactivity

• In Vivo:
  Exogenous bradykinin markedly inhibited TF expression in mRNA and protein level induced by LPS in a dose-dependent manner. Moreover, the NO synthase antagonist L-NAME and PI3K inhibitor LY294002 dramatically abolished the inhibitory effects of bradykinin on tissue factor expression. PI3K/Akt signaling pathway activation induced by bradykinin administration reduced the activity of GSK-3β and MAPK, and reduced NF-x03BA;B level in the nucleus, thereby inhibiting TF expression.Intraperitoneal injection of C57/BL6 mice with bradykinin also inhibited the thrombus formation induced by ligation of inferior vena cava.
• Animal Research:
  To study the effect of bradykinin on TF expression, we used Lipopolysaccharides (LPS) to induce TF expression in human umbilical vein endothelial cells and monocytes. Transcript levels were determined by RT-PCR, protein abundance by Western blotting. In the in vivo study, bradykinin and equal saline were intraperitoneally injected into mice for three days ahead of inferior cava vein ligation that we took to induce thrombus formation, after which bradykinin and saline were injected for another two days. Eventually, the mice were sacrificed and tissues were harvested for tests.

Storage & Handling

• Storage: keep away from moisture | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bradykinin (acetate), Bradykinin, Bradykinin Receptor, BradykininReceptor

Compound Identifiers

PubChem CID

91886389