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Albumin Antibody / Bovine Serum Albumin

SKU: orb2639565

Description

Reacts with a protein of ~66kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2+, Na+, K+, fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1-5ug/ml
ReactivityBovine
Application Notes
Optimal dilution of the Albumin antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.SPM376
ImmunogenPurified bovine serum albumin was used as the immunogen for the Albumin antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Albumin Antibody / Bovine Serum Albumin (orb2639565)

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Available Sizes

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100 μg
$ 750.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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