Bovine IgG (H&L) Antibody

SKU: orb346546

Description

Bovine IgG (H&L) antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine IgG Secondary Antibodies have been tested by SDS-Page and dot blot and are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bovine IgG whole molecule
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 10.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Bovine IgG Antibody, Secondary Antibody

SDS PAGE Results of Goat Anti-Bovine IgG Antibody. Lane 1: Goat Anti-Bovine IgG Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Goat Anti-Bovine IgG Non-Reduced [10 µg]. 4-20% Gel, Coomassie Stained.