Bovine IgG (H&L) Antibody

• Catalog Number: orb346546
• Category: Antibodies
• Description: Bovine IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine
• Form/Appearance: Lyophilized
• Concentration: 10.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum.
• Immunogen: Bovine IgG whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Bovine IgG Secondary Antibodies have been tested by SDS-Page and dot blot and are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Bovine IgG Antibody, Secondary Antibody
• Note: For research use only

SDS PAGE Results of Goat Anti-Bovine IgG Antibody. Lane 1: Goat Anti-Bovine IgG Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Goat Anti-Bovine IgG Non-Reduced [10 µg]. 4-20% Gel, Coomassie Stained.