Bovine IgG Fab Antibody

SKU: orb345992

Description

Bovine IgG Fab Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Bovine IgG F(ab) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Bovine
• Biological Origin: Bovine
• Isotype: IgG Fab
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(ab’)2. No reaction was observed against anti-Bovine IgG F(c) or anti- Papain.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bovine IgG Fab fragment

SDS PAGE Results of Bovine IgG F(ab) Fragment. Lane 1: Bovine IgG F(ab) Reduced 1X. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Bovine IgG F(ab) Non-Reduced 1X. 4-20% Gel, Coomassie Stained.