Biotin (Vitamin B7 or Vitamin H) Antibody

• Catalog Number: orb750924
• Category: Antibodies
• Description: Mouse monoclonal antibody to Biotin (Vitamin B7 or Vitamin H)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BTN399
• Tested applications: FC, IF, IHC, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Biotinylated sheep immunoglobulin
• Concentration: 200 μg/ml
• Dilution range: Confocal Microscopy; In situ Nucleic Acid Hybridization; Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 244Da
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Vitamin B7 or Vitamin H antibody
• Note: For research use only
• Application notes: Positive Control: Biotinylated proteins in solution or on tissues
• Expiration Date: 12 months from date of receipt.