BIN3 Antibody

SKU: orb344478

Description

BIN3 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5000 - 1:50000, IP: 10-100 µL, WB: 1:500-1:1500
• Reactivity: Mouse
• Application Notes:
  Anti-BIN3 antibody has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3A4
• Immunogen: Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.
• Target: BIN3
• Purity: Anti-BIN3 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. BIN3 antibody is specific for human BIN3 protein. A BLAST analysis was used to suggest cross-reactivity with BIN3 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-BIN3 Antibody, Bridging integrator 3, BIN-3, BIN 3, BIN3 antibody, anti-BIN-3 antibody

Western Blot of Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 µg per lane. Primary antibody: BIN3 antibody overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band(s): non-specific.

Western Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 µg per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics.

Quick Database Links

Gene Symbol

BIN3

UniProt

Q9NQY0

RefSeq (Protein):NP_061158.1