BIN3 Antibody

SKU: orb344477

Description

BIN3 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5000-1:50000, IP: 10-100 µL, WB: 1:500-1:1500
• Reactivity: Mouse
• Application Notes:
  Anti-BIN3 antibody has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3A4
• Immunogen: Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.
• Target: BIN3
• Purity: Anti-BIN3 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. BIN3 antibody is specific for human BIN3 protein. A BLAST analysis was used to suggest cross-reactivity with BIN3 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-BIN3 Antibody, Bridging integrator 3, BIN-3, BIN 3, BIN3 antibody, anti-BIN-3 antibody

Western Blot of Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 µg per lane. Primary antibody: BIN3 antibody overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band(s): non-specific.

Western Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 µg per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics.

Quick Database Links

Gene Symbol

BIN3

UniProt

Q9NQY0

RefSeq (Protein):NP_061158.1