betaCstF-64 variant 1/ betaCstF-64 variant 3 Antibody

SKU: orb1248316

Description

betaCstF-64 variant 1/ betaCstF-64 variant 3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IP
• Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Immunoprecipitation: In transfected HeLa transiently expressing Mouse ßCstf-64 ((DYKDDDDK tagged), a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2ug per lysate. Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed ßCstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both ßCstf-64 anf Cstf-64 Recommended amount 2ug EB11838/500ug lysate protein.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PASIERVQGQRT
• Target: betaCstF-64 variant 1/ betaCstF-64 variant 3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cstf2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64kDa, C630034J23Rik, Cstf64, CF-1 64 kDa subunit, CSTF 64 kDa subunit, alphaCstF-64 variant 4, betaCstF-64 variant 1, betaCstF-64 variant 3, cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa, cstF-64

HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using orb1248316 (2 ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDD.

orb1248316 (2 ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500 ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500 ug) protein) using Protein G-coated magnetic beads.

Quick Database Links

Gene Symbol

betaCstF-64 variant 1/ betaCstF-64 variant 3

UniProt

Q8BIQ5

RefSeq:ACF05702.1, ACF05700.1