FBXW11 Antibody

SKU: orb345450

Description

Beta TrCP2 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 – 1:100,000, WB: 1:200 to 1:1,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.
• Target: FBXW11
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human ßTrCP2 protein. A BLAST analysis was used to suggest cross-reactivity with ßTrCP2 from human, mouse, dog, bovine, chimpanzee, chicken and horse based on a 100% homology with the immunizing sequence. Cross-reactivity with ßTrCP2 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.8 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-beta TrCP2 antibody, rabbit anti-BTRCP2 antibody, F-box/WD repeat-containing protein 11, beta Transducin Repeat Containing Protein 2 antibody, BTRC2 antibody, F-box and WD-40 Domain Protein 1B antibody, F-box Protein FBW1B antibody, FBW1B antibody, FBXW1B antibody, Homologous to Slimb protein, HOS, FBXW11

Western blot using Biorbyt's affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (p/n orb348714) [lane 1] and 293 (p/n orb348669) [lane 2] whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n orb347654) diluted 1:20000 in blocking buffer (p/n orb348637) for 1 h at 4 °C.

Quick Database Links

Gene Symbol

FBXW11

UniProt

Q9UKB1

RefSeq:48928048