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beta Catenin Antibody, KO Validated

Catalog Number: orb1274541

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1274541
CategoryAntibodies
Descriptionbeta Catenin Antibody, KO Validated
TargetCTNNB1
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
Concentrationbatch dependent
Buffer/PreservativesLiquid
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 650-750 of human beta Catenin (NP_001895.1).
UniProt IDP35222
MWObserved: 105kDa
Tested applicationsIHC, IP, WB
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCTNNB1, CTNNB, MRD19, armadillo
NoteFor research use only
beta Catenin Antibody, KO Validated

Western blot analysis of extracts of various cell lines, using beta Catenin antibody (orb1274541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

beta Catenin Antibody, KO Validated

Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) 293T cells, using beta Catenin antibody (orb1274541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded rat heart using beta Catenin antibody (orb1274541) at dilution of 1:100 (40x lens).

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded mouse liver using beta Catenin antibody (orb1274541) at dilution of 1:100 (40x lens).

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded mouse heart using beta Catenin antibody (orb1274541) at dilution of 1:100 (40x lens).

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded rat fallopian tube using beta Catenin antibody (orb1274541) at dilution of 1:150 (40x lens).

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded human colon carcinoma using beta Catenin antibody (orb1274541) at dilution of 1:150 (40x lens).

beta Catenin Antibody, KO Validated

Immunohistochemistry of paraffin-embedded human stomach using beta Catenin antibody (orb1274541) at dilution of 1:150 (40x lens).