APP Antibody

SKU: orb345740

Description

Beta Amyloid pyro E3 antibody

Images & Validation

• Tested Applications: DOT, ELISA, IF, IHC, Multiplex Assay, WB
• Dilution Range: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 1 µg/ml
• Reactivity: Human
• Application Notes:
  Anti-Beta Amyloid pyro Glu3 has been tested in dot blot, ELISA, Western Blot, and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 3-pyro E start point of human beta Amyloid.
• Target: APP
• Purity: Anti-Beta Amyloid pyro Glu3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody contains no reactivity towards the 1-42 ABeta peptide. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Bovine and Sheep based on 100% sequence homology. It is not reactive with Rodentia. Cross-reactivity with beta Amyloid pyro Glu3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Beta Amyloid pyro E3 Antibody, ß-amyloid pyro E3, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

Immunofluorescence Microscopy of Rabbit Anti-beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 µg/ml for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: ß Amyloid pyro E3 as green fluorescent signal, ß Amyloid 3 as yellow signal; and Bottom: ß Amyloid 3 as red signal with co-incubation of ß Amyloid pyro E3 peptide.

Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 µg per lane. Primary antibody: ß Amyloid pyro E3 antibody at 2 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.

Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 µg per lane 1-2, 0.2 µg per lane 3-4, 0.05 µg per lane 5-6, 0.01 µg per lane 7-8, 0.005 µg per lane 9-10. Primary antibody: ß Amyloid pyro E3 antibody at 0.5 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with ß Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the ß Amyloid 42 Aβ peptide (data not shown).

Quick Database Links

Gene Symbol

APP

UniProt

P05067

RefSeq (Protein):NP_000475.1