APP Antibody

SKU: orb345371

Description

Beta Amyloid antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:30,000, IHC: 1:50-1:200, IF: 1:50-1:200, WB: 1:1,000-1:5000
• Reactivity: Human, Mouse
• Application Notes:
  Affinity purified anti-beta amyloid has been tested by ELISA, IHC, WB, and IF. A 45.8kDa band is detected in western blot using whole tissue extracts and lysates from mouse and human. In general, we recommend the use of 4% PFA or 10% formalin for fixation of tissues with IHC-paraffin or IHC-frozen application of this antibody.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.
• Target: APP
• Purity: This affinity purified antibody is directed against extracellular region of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Blast analysis of the sequence of the immunogen shows 100% identity with Human, Guinea Pig, Pig, Cyno Monkey, Dog, Polar Bear, Rabbit, Chimp, Squirrel monkey, and Sheep. Cross reactivity with beta amyloid from other species is likely but has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Beta Amyloid Antibody, ß-amyloid, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Beta-amyloid precursor protein

Quick Database Links

Gene Symbol

APP

UniProt

P05067

RefSeq (Protein):NP_000475.1