BCMA Antibody

• Catalog Number: orb1239053
• Category: Antibodies
• Description: BCMA Antibody
• Target: TNFRSF17
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: BCMA Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: BCMA Antibody is affinity chromatography purified via peptide column.
• Immunogen: BCMA antibody was raised against a 16 amino acid synthetic peptide mapping at the carboxy terminus of human BCMA.The immunogen is located within the last 50 amino acids of BCMA.
• UniProt ID: Q02223
• MW: Predicted: 20 kDa Observed: 27 kDa
• Tested applications: ELISA, IF, IHC-P, WB
• Application notes: BCMA antibody can be used for detection of BCMA by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BCMA Antibody: BCM, BCMA, CD269, TNFRSF13A, BCM, T
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001183

Immunofluorescence Validation of BCMA in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling BCMA with orb1239053 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of BCMA in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BCMA antibody (orb1239053) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of BCMA in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BCMA antibody (orb1239053) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

WB Validation in Human Tissues. Loading: 10 µg of lysate Antibodies: BCMA, orb1239053, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.