Basophils, Human, mAb 2D7 Antibody

SKU: orb1108851

Description

Mouse monoclonal antibody to Basophils

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, PS, WB
• Dilution Range: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Reactivity: Human
• Application Notes:
  IF: Cytospin preparations were fixed in methanol for 15 min, incubated in methanol containing 0.6% H2O2, for 30 min to block endogenous peroxidase, washed in dH2O, incubated with goat serum (1/500 dilution) for 2 h to block nonspecific staining. Blocked slides were washed with TTBS, pH7.4. (Ref.1) FC: Antibody 2D7 stains the extracellular domain of basophils. As negative control CD203c negative cells (basophil depleted cell fractions) (Ref.3) W: A reduced sample treatment and SDS-Page was used. The band sizes are 72 en 76 kDa (Ref.1). IHC-P: Fresh surgical tissues were fixed in Carnoy's fluid for 24 h and transferred to absolute ethanol. Tissues were embedded in paraffin, and 4-um sections were prepared. Tissue sections were dewaxed in xylene and rehydrated in graded ethanol solutions. Endogenous peroxidase was inhibited. (Ref.1) IHC-F: Sections were fixed in either 10% NBF or Carnoy's fluid for 15 minutes at RT. NBF-fixed tissue sections were digested with 0.1% protease in TTBS for 20 min at RT. Endogenous peroxidase activity was inhibited by methanol with 0.6% H2O2 for 30 min at RT. Blocked by incubation in horse serum at 1:20 dilution in TTBS for 1 hr at RT. 2D7 at a 1:300 dilution in TTBS overnight at 4deg C or with a murine mAb of undetermined specificity (MOPC31-C) as a negative control. (Ref.2)

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Lysate of purified basophils
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only