Avicularin

SKU: orb1222019

Description

Avicularin exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 macrophage cells. Avicularin inhibits the accumulation of the intracellular lipids by decreasing C/EBPα-activated GLUT4-mediated glucose uptake in adipocytes and potently inhibiting fatty acid synthase.

Key Properties

• CAS Number: 572-30-5
• MW: 434.35
• Purity: >98% (HPLC)
• Formula: C₂₀H₁₈O₁₁
• SMILES: C1=CC(=C(C=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)OC4C(C(C(O4)CO)O)O)O)O
• Target: ERK
• Solubility: In Vitro: DMSO : 100 mg/mL (230.23 mM)

Bioactivity

• In Vivo:
  Animal model: ACLT (anterior cruciate ligament transection)-induced rats. Dosage: 0.5, 1, 2 mg/kg. Administration: Injected into the articular cavity of the right knee, twice a week for 4 weeks. Result: Alleviated the tibial subchondral osteolysis, reduces bone loss, and increases the bone mass of tibial subchondral bone. Attenuated ECM degradation, the loss of Aggrecan and Collagen II in ACLT-induced rats. Decreased the MMP3 and MMP13 protein level. Animal model: Rats with amyloid Beta-induced Alzheimer's disease. Dosage: 25, 50, and 100 mg/kg. Administration: Oral administration, for 21 days. Result: Enhanced cognition activity, and reversed the effects of amyloid beta-induced inflammatory response and excessive oxidative stress.
• In Vitro:
  Cell Proliferation Assay Cell line: Huh7 cells. Concentration: 25, 50, 100 μg/mL. Incubation time: 12, 24, 36 and 48 h. Result: Inhibited cell proliferation in a dose-dependent manner. Western blot analysis. Cell line: RAW 264.7 cells Concentration: 10, 30, 100, 300 μM. Incubation time: 1 h. Result: Inhibited LPS-induced protein expression of iNOS and COX-2, release of pro-inflammatory cytokine IL-1β, degradation of cytosolic IκB, and phosphorylation of ERK. RT-PCR Cell line: 3T3-L1 cells. Concentration: 50 μM. Incubation time: 6 days. Result: Decreased PPARγ, C/EBPα, and aP2 mRNA levels approximately 28.7, 69.5, and 18.3%, respectively.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

ERK