ATP6V0D2 Rabbit Polyclonal Antibody

SKU: orb325998

Description

Rabbit polyclonal antibody to ATP6V0D2

Images & Validation

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Item 1 of 4

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human ATP6V0D2
Target	ATP6V0D2
Molecular Weight	40 kDa
Purification	Affinity Purified

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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anti ATP6D2 antibody, anti FLJ38708 antibody, anti VMA6 antibody

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25 ug of the indicated Human tumor tissue cell extracts was loaded onto a 12% SDS-PAGE gel. 2 ug/mL of the antibody was used in this experiment. This protein seems to migrate higher than it's amino acid sequence predicts.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-ATP6V0D2 Antibody Titration: 0.2-1 ug/mL. A: 5 ug empty vector. B: 5 ug hATP6V0D2 transfected HEK293T.

WB Suggested Anti-ATP6V0D2 Antibody Titration: 0.2-1 ug/mL. ELISA Titer: 1:62500. Positive Control: Transfected 293T.

Quick Database Links

Gene Symbol

ATP6V0D2

UniProt

Q8N8Y2

RefSeq (Protein):NP_689778

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_689778

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Protocol Information

Western Blot (IB, immunoblot)

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