ATF7 Antibody (N-term)

SKU: orb1928249

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21430
• Target: This ATF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human ATF7.
• Molecular Weight: 51757 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A, ATF7, ATFA

ATF7 Antibody (N-term) western blot analysis in huamn placenta tissue lysates (35 ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow).

ATF7 Antibody (N-term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Quick Database Links

Gene Symbol

This ATF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human ATF7.

UniProt

P17544

RefSeq (Protein):NP_001193611.1, NP_001123532.1, NP_001193612.1, NP_006847.1