Armenian Hamster IgG (H&L) Secondary Antibody Biotin Conjugated

• Catalog Number: orb348066
• Category: Antibodies
• Description: Armenian Hamster IgG (H&L) antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Hamster
• Isotype: IgG
• Immunogen: Armenian Hamster IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Reactivity with Golden Syrian Hamster IgG is greatly reduced.
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Armenian Hamster IgG Antibody biotin Con
• Note: For research use only
• Application notes: Biotin Anti-Hamster IgG Secondary Antibody has been tested by ELISA and western blot and is designed for Western Blotting, ELISA and Immunohistochemistry. Biotin conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
• Expiration Date: 12 months from date of receipt.

Western Blot of Biotin conjugated Goat anti-Armenian Hamster IgG antibody. Lane 1: Armenian Hamster IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: Armenian Hamster IgG Biotin conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Human IgG. Other band(s): none.