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Catalog Number | orb348064 |
---|---|
Category | Antibodies |
Description | ARMENIAN HAMSTER IgG (H&L) (GOAT) antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Hamster |
Isotype | IgG |
Immunogen | Armenian Hamster IgG whole molecule |
Antibody Type | Secondary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Greatly diminished reactivity will occur against Golden Syrian Hamster IgG. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | goat anti-Armenian Hamster IgG Antibody peroxidase Read more... |
Note | For research use only |
Application notes | Anti-Hamster IgG HRP Secondary Antibody has been tested by ELISA and is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development. |
Expiration Date | 12 months from date of receipt. |
ELISA results of purified Goat anti-Armenian Hamster IgG Antibody tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n orb346446). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.