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Item 1 of 4
ARHGDIA Antibody (C-term)
Catalog Number: orb1931355
| Catalog Number | orb1931355 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20512 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 23207 Da |
| Target | This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human ARHGDIA. |
| UniProt ID | P52565 |
| NCBI | NP_001172007.1, NP_001172006.1, NP_004300.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-G Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ARHGDIA Antibody (C-term) in A375 (lane 1), HL-60 (lane 2), Ramos (lane 3) cell line lysates (35 ug/lane). ARHGDIA (arrow) was detected using the purified Pab.
Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
