APC1 (phospho-S377) antibody

• Catalog Number: orb345507
• Category: Antibodies
• Description: APC1 (phospho-S377) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.
• Concentration: 0.21 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 5.0 µg/ml, WB: 1:200 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
• Conjugation: Unconjugated
• UniProt ID: Q9H1A4
• NCBI: 12056971
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANA
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human multi-brain and multi-cancer tissue using APC1 (phospho-S377) antibody

Western blot analysis of shows lysate from asynchronous cells (Lane1), shows lysate from cells treated with nocodazole (Lane2) using APC1 (phospho-S377) antibody