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Anti-TCP1 delta/CCT4 Antibody

Catalog Number: orb371661

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb371661
CategoryAntibodies
DescriptionAnti-TCP1 delta/CCT4 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC, IHC-Fr, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW58 kDa
UniProt IDP50991
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesT-complex protein 1 subunit delta; TCP-1-delta; CC
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-TCP1 delta/CCT4 Antibody

Flow Cytometry analysis of K562 cells using anti-CCT4 antibody. Overlay histogram showing K562 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-TCP1 delta/CCT4 Antibody

Flow Cytometry analysis of PC-3 cells using anti-CCT4 antibody. Overlay histogram showing PC-3 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-TCP1 delta/CCT4 Antibody

Flow Cytometry analysis of U251 cells using anti-CCT4 antibody. Overlay histogram showing U251 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-TCP1 delta/CCT4 Antibody

IF analysis of CCT4 using anti-CCT4 antibody. CCT4 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-CCT4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of CCT4 using anti-CCT4 antibody. CCT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CCT4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of CCT4 using anti-CCT4 antibody. CCT4 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CCT4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of CCT4 using anti-CCT4 antibody. CCT4 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CCT4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in frozen section of mouse brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in frozen section of rat brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in immunocytochemical section of LOVO cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in immunocytochemical section of Neuro-2a cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in immunocytochemical section of NRK cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

IHC analysis of TCP1 delta using anti-TCP1 delta antibody. TCP1 delta was detected in immunocytochemical section of SHG-44 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-TCP1 delta Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-TCP1 delta/CCT4 Antibody

Western blot analysis of CCT4 using anti-CCT4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCT4 at approximately 58 kDa. The expected band size for CCT4 is at 58 kDa.

  • Anti-TCP1 delta/CCT4 Antibody [orb2618166]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-TCP1 delta/CCT4 Antibody [orb2618167]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-TCP1 delta/CCT4 Antibody [orb2618168]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-TCP1 delta/CCT4 Antibody [orb2618169]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-TCP1 delta/CCT4 Antibody [orb2618170]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    FITC

    100 μg