SPT6 Rabbit Polyclonal Antibody

SKU: orb668318

Description

The SPT6 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human SPT6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human SPT6. The exact sequence is proprietary.
• Target: SUPT6H
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIAA0162; SPT6H; Transcription elongation factor SPT6; hSPT6; Histone chaperone suppressor of Ty6; Tat-cotransactivator 2 protein; Tat-CT2 protein

Western blot analysis of SPT6 expression in HeLa (A), mouse brain (B) whole cell lysates. (Predicted band size: 61; 84; 199 kD; Observed band size: 260 kD)

Immunofluorescent analysis of SPT6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

SUPT6H

UniProt

Q62383, Q7KZ85

NCBI Gene ID

20926, 6830