Anti-RRM2 [SAIC-30C-18]

• Catalog Number: orb1089986
• Category: Antibodies
• Description: Rabbit monoclonal antibody to RRM2
• Target: RRM2
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The KLH-conjugated peptide "IEQEFLTEALPVK" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.
• UniProt ID: P31350
• Tested applications: ELISA, WB
• Clone Number: SAIC-30C-18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RR2; R2; RR2M; ribonucleotide reductase regulatory
• Research Area: Epigenetics
• Note: For research use only

Flow cytometry using the Anti-RRM2 antibody SAIC-30C-18. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (orb1089986, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-RRM2 SAIC-30C-18. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (orb1089986) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089986, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-RRM2 antibody SAIC-30C-18. Jurkat cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (orb1089986) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.