PKAc Antibody

SKU: orb43621

Description

Mouse Monoclonal to PKAc.

Research Area

Epigenetics

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 6D2.1
• Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
• Target: PKAc
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKACA, PPNAD4, PRKACA, Proteinkinase Ac

Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. orb44500). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1; Lane 2: GST-Akt2; Lane 3: GST-Akt3; Lane 4: GST-PKAc alpha; Lane 5: GST-PKAc beta; Lane 6: GST-PKAc gamma; Lane 7: GST-MEK 1; Lane 8: GST.

Quick Database Links

Gene Symbol

PKAc

UniProt

P17612

NCBI Gene ID

5566