Peroxidase (Mouse) Antibody

SKU: orb344554

Description

Peroxidase (Mouse) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:3,000
• Application Notes:
  Anti-Peroxidase (Horseradish) (Mouse) antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, conjugation, and most immunological methods requiring high titer and specificity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Peroxidase [Horseradish]
• Purity: Anti-Peroxidase (horseradish) (Mouse) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse anti-peroxidase antibody, mouse anti-HRP, Horseradish peroxidase

Western Blot of Mouse anti-Peroxidase (Horseradish) Antibody. Lane 1: Peroxidase (Horseradish). Load: 100 ng per lane. Primary antibody: Mouse anti-Peroxidase (Horseradish) Antibody at 1:1000 overnight at 4°C. Secondary antibody: Fluorescein conjugated mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 44 kDa, 44 kDa for Horseradish Peroxidase.