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Description
Images & Validation
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| Tested Applications | ICC, IF, WB |
|---|---|
| Dilution Range | Western blots of yeast protein samples: 1:5,000 (cell lysates) to 1:12,500 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. For immunofluorescence on yeast cells: 1:1,000 to 1: 10,000. |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | B40H4 |
| Immunogen | Raised against Yeast (S. cerevisiae) fibrillarin |
| Purity | Monoclonal |
| Purification | Monoclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration | 1ug/ul |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
Anti-Nop1p (orb1109259)
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